The wide tuning range, high power and short pulses of the primary output is ideal for optimum excitation of all popular fluorophores (inclusive of eGFP, YFP and all the red-shifted proteins). Just as important, Chameleon Discovery‘s secondary output at 1040 nm provides high power, short pulses (160 fs) and excellent beam parameters, truly enabling a new era of simultaneous, multi-wavelength excitation of multiple probes and/or photoactivation of opsins for optogenetics applications. These applications include optogenetic experiments based on established schemes using longer wavelength opsins like C1V1, as well as alternative wavelength pairings based on red-shifted calcium indicators of the GCaMP family currently under development. The availability of dual wavelength pulses that are phase-correlated makes Chameleon Discovery also a perfect source for techniques like CARS and SRS.